Jodi Craigo, PhD

Research Associate Professor


Dr. Craigo

Contact

412-648-8874
Fax: 412-624-4440

9017 Biomedical Science Tower 3

3501 Fifth Avenue

Pittsburgh, PA 15261-1308

Education

PhD in Cellular & Molecular Biology, Northeastern Ohio Universities, College of Medicine

Research Summary

My research started in the characterization of lentiviral persistence and pathogenesis, conducted through evolutionary studies of both HIV and EIAV sequence analysis. Our evolutionary analysis of lentiviral populations via quasispecies sequence analysis included determinations of phenotypic differences of conserved and immunodominant regions of protein variation utilizing multiple computational avenues including: phylogenetics, divergence and selection analyses, and compartmentalization analyses (including Slatkin-Maddison, Mantel and GeneFlow computations). A major focus of our lentiviral research in the last decade was animal models of AIDS vaccines. Our lentiviral vaccine development included various modalities such as live attenuated, viral vector and virus like particle vaccines. Our vaccine research applied what we learn from pathogenesis studies into mechanisms of protection and results ultimately in the implementation of the new candidate vaccines into trials. In addition to the development of vaccine candidates, we also focused on the creation of new, rigorous virus challenge models. A major focus of my independent research focused on the detailed characterization of dynamic antigen-antibody interactions that included analyzing protective/non-protective immune system responses; specifically utilizing SPR analysis for the development of novel immune assays and characterizations of the structure:function relationship to correlates of protection. Most recently my research has shifted focus, to:

1) the development of antimicrobial peptides against drug-resistant bacteria and utilizing SPR to dissect peptide-membrane interactions and evaluate mechanisms of efficacy; and,

2) the development of high specificity, high-throughput, time-resolving peptoid-based diagnostics for Zika Virus infection.

Click here for a full listing of publications

Research Lab Affiliation

Publications

Deslouches B, Steckbeck JD, Craigo JK, Doi Y, Burns JL and Montelaro RC. 2015. Engineered cationic antimicrobial peptides to overcome multidrug resistance by ESKAPE pathogens. Antimicrob Agents Chemother. 59: 1329-1333. |  View Abstract

Craigo JK, Ezzelarab C, Cook SJ, Chong L, Horohov D, Issel CJ and Montelaro RC. 2015. Protective Efficacy of Centralized and Polyvalent Envelope Immunogens in an Attenuated Equine Lentivirus Vaccine. PLoS Pathog. 11: e1004610. doi: 10.1371/journal.ppat.1004610. |  View Abstract

Deslouches B, Steckbeck JD, Craigo JK, Doi Y, Mietzner TA and Montelaro RC. 2013. Rational design of engineered cationic antimicrobial peptides consisting exclusively of arginine and tryptophan, and their activity against multidrug-resistant pathogens. Antimicrob Agents Chemother. 57: 2511-2521. |  View Abstract

Craigo JK, Ezzelarab C, Cook SJ, Chong L, Horohov D, Issel CJ and Montelaro RC. 2013. Envelope determinants of equine lentiviral vaccine protection. PLoS One. 8: e66093. |  View Abstract

Craigo JK, Zhang B, Barnes S, Tagmyer TL, Cook SJ, Issel CJ and Montelaro RC. 2007. Envelope variation as a primary determinant of lentiviral vaccine efficacy. Proc Natl Acad Sci USA. 104: 15105-15110. |  View Abstract