Jerrod A. Poe, PhD

Research Assistant Professor


headshot

Contact

412-648-9240
Fax: 412-624-1401

533 Bridgeside Point II

450 Technology Drive

Pittsburgh, PA 15219

Education

PhD in Biochemistry and Molecular Genetics, University of Pittsburgh, School of Medicine, 2009

BS in Biology, Erskine College, 2003

Research Summary

My research focuses on (1) The development and implementation of high-throughput/high-content screening assays for the discovery of small molecules that influence the quaternary structure of the HIV-1 Nef virulence factor.  (2) Executing the confirmatory and secondary (functional) assays required to validate potential inhibitors identified from the primary screening process.  Testing includes monitoring levels of HIV replication, infectivity, receptor downregulation and key protein-protein interactions. 

Following his graduation, Jerrod received an offer to work under his mentor, Dr. Tom Smithgall, as a Postdoctoral Associate.

Mentor

Thomas E. Smithgall, PhD

Dissertation

Research Lab Affiliation

Publications

Tarafdar S, Poe J.A. and Smithgall, T.E. (2014) The accessory factor Nef links HIV-1 to Tec/Btk kinases in an Src homology 3 domain-dependent manner. J Biol Chem. 289:15718-15728. |  View Abstract

Poe J.A., Vollmer L, Vogt A and Smithgall, T.E. (2014) Development and validation of a high-content bimolecular fluorescence complementation assay for small-molecule inhibitors of HIV-1 Nef dimerization. J Biomol Screen. 19: 556-565. |  View Abstract

Perrone R; Nadai M; Poe J.A; Frasson I; Palumbo M; Palu G; Smithgall T.E. and Richter, S.N. (2013) Formation of a unique cluster of G-quadruplex structures in the HIV-1 Nef coding region: implications for antiviral activity. PLoS One 8: e73121. |  View Abstract

Perrone R; Nadai M; Frasson I; Poe J.A; Butovskaya E; Smithgall T.E; Palumbo M; Palu G and Richter S.N. (2013) A dynamic G-quadruplex region regulates the HIV-1 long terminal repeat promoter. J Med Chem. 56: 6521-6530. |  View Abstract

Emert-Sedlak L.A; Narute P; Shu S.T; Poe J.A; Shi H; Yanamala N; Alvarado J.J; Lazo J.S; Yeh J.I; Johnston P.A. and Smithgall T.E. (2013) Effector kinase coupling enables high-throughput screens for direct HIV-1 Nef antagonists with antiretroviral activity. Chem Biol. 20: 82-91. |  View Abstract

Poe J.A and Smithgall T.E. (2009) HIV-1 Nef dimerization is required for Nef-mediated receptor downregulation and viral replication. J Mol Biol. 394: 329-342. |  View Abstract

Ye H; Choi H.J; Poe J and Smithgall T.E. (2004) Oligomerization is required for HIV-1 Nef-induced activation of the Src family protein-tyrosine kinase, Hck. Biochemistry. 43: 15775-15784. |  View Abstract